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Abstract In simulations of radiative‐convective equilibrium (RCE), and with sufficiently large domains, organized convection enhances top of atmosphere outgoing longwave radiation due to the reduced cloud coverage and drying of the mean climate state. As a consequence, estimates of climate sensitivity and cloud feedbacks may be affected. Here, we use a multi‐model ensemble configured in RCE to study the dependence of explicitly calculated cloud feedbacks on the existence of organized convection, the degree to which convection within a domain organizes, and the change in organized convection with warming sea surface temperature. We find that, when RCE simulations with organized convection are compared to RCE simulations without organized convection, the propensity for convection to organize in RCE causes cloud feedbacks to have larger magnitudes due to the inclusion of low clouds, accompanied by a much larger inter‐model spread. While we find no dependence of the cloud feedback on changes in organization with warming, models that are, on average, more organized have less positive, or even negative, cloud feedbacks. This is primarily due to changes in cloud optical depth in the shortwave, specifically high clouds thickening with warming in strongly organized domains. The shortwave cloud optical depth feedback also plays an important role in causing the tropical anvil cloud area feedback to be positive which is directly opposed to the expected negative or near zero cloud feedback found in prior work. 

The primate dorsolateral prefrontal cortex (DLPFC) displays unique in vivo activity patterns, but how in vivo activity regulates DLPFC pyramidal neuron (PN) properties remains unclear. We assessed the effects of in vivo Kir2.1 overexpression, a genetic silencing tool, on synapses in monkey DLPFC PNs. We show for the first time that recombinant ion channel expression successfully modifies the excitability of primate cortex neurons, producing effects on synaptic properties apparently different from those in the rodent cortex. 

Institutions of Higher Education are complex matrixed organizations that require collaboration across silos. Research Administration, Institutional Research, Enterprise IT, and Research Computing are important areas to leverage when building a research analytics program. Across these units, our work can look very different, and we likely have different mental models for how we think about and approach work. This can make cross-functional communication and collaboration difficult. This presentation will provide a mental model for working in higher education research analytics, discuss critical players, and how to get work moving forward. Finally, we will provide a list of key ingredients to consider when building your institution’s research analytics program and strategies for effective buy-in and collaboration. 

Abstract Radiative‐convective equilibrium (RCE) is particularly well suited for studying tropical deep‐convection, a regime of clouds that contributes some of the highest uncertainties to the estimates of total cloud feedback. In order to perform a comprehensive calculation and decomposition of cloud feedbacks in cloud‐permitting models, previously primarily done in global climate models, the configuration of a satellite simulator for use with offline data was successfully implemented. The resultant total cloud feedback is slightly positive, primarily driven by the longwave effects of increases in cloud altitude. The high‐cloud altitude feedback is robustly positive and has a central value and uncertainty well‐matched with prior estimates. Reductions in high cloud amount drive a tropical anvil cloud area feedback that is on average negative, consistent with prior estimates. However, a subset of models with finer horizontal grid spacing indicate that a positive tropical anvil cloud area feedback cannot be ruled out. Even though RCE is only applicable to tropical deep‐convective clouds, the RCE total cloud feedback is within the range of prior comprehensive estimates of the global total cloud feedback. This emphasizes that the tropics heavily influence the behavior of global cloud feedbacks and that RCE can be exploited to learn more about how processes related to deep convection control cloud feedbacks. 

Abstract Biodiversity indicators are used to assess progress towards conservation and sustainability goals. However, the spatial scales, methods and assumptions of the underlying reporting metrics can affect the provided information. Using mountain ecosystems as an example, we compare biodiversity protection at subnational scale using the site-based approach of the 2030 Agenda for Sustainable Development (SDG indicator 15.4.1) with an area-based approach compatible with the targets of the Kunming–Montreal Global Biodiversity Framework. 

Understanding metabolism in the pathogen Candida glabrata is key to identifying new targets for antifungals. The thiamine biosynthetic (THI) pathway is partially defective in C . glabrata , but the transcription factor Cg Pdc2 upregulates some thiamine biosynthetic and transport genes. One of these genes encodes a recently evolved thiamine pyrophosphatase ( CgPMU3 ) that is critical for accessing external thiamine. Here, we demonstrate that Cg Pdc2 primarily regulates THI genes. In Saccharomyces cerevisiae , Pdc2 regulates both THI and pyruvate decarboxylase (PDC) genes, with PDC proteins being a major thiamine sink. Deletion of PDC2 is lethal in S . cerevisiae in standard growth conditions, but not in C . glabrata . We uncover cryptic cis elements in C . glabrata PDC promoters that still allow for regulation by Sc Pdc2, even when that regulation is not apparent in C . glabrata . C . glabrata lacks Thi2, and it is likely that inclusion of Thi2 into transcriptional regulation in S . cerevisiae allows for a more complex regulation pattern and regulation of THI and PDC genes. We present evidence that Pdc2 functions independent of Thi2 and Thi3 in both species. The C-terminal activation domain of Pdc2 is intrinsically disordered and critical for species differences. Truncation of the disordered domains leads to a gradual loss of activity. Through a series of cross species complementation assays of transcription, we suggest that there are multiple Pdc2-containing complexes, and C . glabrata appears to have the simplest requirement set for THI genes, except for CgPMU3 . CgPMU3 has different cis requirements, but still requires Pdc2 and Thi3 to be upregulated by thiamine starvation. We identify the minimal region sufficient for thiamine regulation in CgTHI20 , CgPMU3 , and ScPDC5 promoters. Defining the cis and trans requirements for THI promoters should lead to an understanding of how to interrupt their upregulation and provide targets in metabolism for antifungals.